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Samples were prepared with Laemmeli sample buffer, and loaded into BioRad mini-protean II electrophoresis system at 150v until dye ran to end

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1 H NMR and 13 C NMR spectra were recorded at 300 and 75 MHz using a Brker AC300 instrument and at 600 MHz using a Brker AC600 instrument

doi: 10.3390/biom13101512 15 BokoviM.VovkT.Kores PlesniarB.GrabnarI