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Male BALB/c-nu nude mice aged 4 weeks and weighing 18 ~ 22 g were purchased from the Experimental Animal Centre of Southern Medical University (Guangzhou, China) and housed in a specific pathogen-free air-conditioned room (25 2 and 65% 5% humidity) with a 12-h light/dark cycle

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It serves as a primary line of defense against cellular wear and tear and supports the natural detoxification pathways in the liver

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Combined these results indicated that NP4 catalyzes the generation of H 2 O 2 in tumor cells, which diffuses outside the cells and activates macrophages, thereby triggering immune responses