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GSH biosynthesis occurs exclusively in the cytosol via a two-step ATP-dependent reaction catalysed sequentially by two enzymes: gamma-glutamylcysteine synthetase (GCL also referred to as gamma-glutamylcysteine ligase, comprising the catalytic GCLC subunit and regulatory GCLM subunit), which catalyses the rate-limiting first step of joining glutamate and cysteine
As of right now, the benefits are unsubstantiated
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MTHFR deficiencies lead to a shortage of the active form of folate within the body and negatively impacts the conversion of homocysteine to methionine
Maintenance doses are then tailored to keep those levels topped up, and their duration of effect is directly tied to the prescribed frequency (e.g., a shot every 12 weeks for many)
