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Use this formula: Concentration (mg/mL) = Vial Size (mg)BAC Water (mL) Example: a 10 mg vial reconstituted with 2 mL of BAC water yields 5 mg/mL
The strongest evidence is in animal research
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10.1111/j.1467-7652.2010.00510.x
Cells were passaged into a new 6-well plate at a density of 1.2 10 6 cells per well, ~30 min before transfection in 2 mL of 2i medium and transfected with 250 L of Opti-MEM containing 8 L of Lipofectamine 3000 and 46 g of plasmid DNA
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