In the initial larger, higher resolution, dataset of Cagri-CTR-Gs ( N = 1), density was observed for the proximal region of the conjugated lipid at the peptide N-terminus that was consistent regardless of peptide conformation (bypass or CT-like) and supports modelling the Glu linker and a short segment of acyl chain in a different orientation to that observed in the AMY 1 R complex (Figs
The reduction of DTNB by non-oxidized thiols forms a yellow derivative (5-thio-2-nitrobenzoate), which was measured at 412 nm, providing the total thiol content in the sample
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