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26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer
Journal of Investigative Dermatology, 113(3), 364-368
doi: 10.1107/S0907444912001308 3 Ayoub MoubareckC.Hammoudi HalatD
(PubMed) Meplan C, Rohrmann S, Steinbrecher A, et al
These are uncomfortable but generally manageable if dosing is monitored
Syringe units volume 100 For insulin units, 100 units equals 1 mL, so mL is multiplied by 100
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