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After the study, all models were histologically examined

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Future studies should incorporate AUC-based assessments to better quantify hormonal dynamics postoperatively and their relationship to clinical outcomes

Once the brown preadipocytes reached 7080% confluence, they were infected with retroviral particles encoding SV40 Large T antigen in culture medium containing 20% heat-inactivated fetal bovine serum (FBS) and polybrene (8 g/mL) for 48 h