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Actin staining was then performed with pre-conjugated Phalloidin-Alexa Fluor 488 (Cytoskeleton, PHDG1A) diluted 1:140 in Antibody Diluent Reagent Solution (Invitrogen, 003218) for 30 min in the dark at room temperature

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In in vivo preclinical models, the route of administration, dosing interval, and the specific wound model chosen will significantly affect outcomes