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After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter

Under conditions of mitochondrial stress, PINK1 accumulates on the outer mitochondrial membrane, recruiting Parkin, which ubiquitinates mitochondrial proteins (Fig

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