Cancer Res 2004
202,203 Essentially, the imported disulphide-containing proteins undergo cathepsin-catalysed degradation in the lysosome, leading to the formation of cystine, which is then effluxed by the cystinosin transporter into the cytosol, where it is reduced to cysteine in a process that requires NADPH, leading to the formation of oxidised glutathione
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Several factors, including aging, stress, inadequate nutrition, and exposure to environmental toxins, can lead to a reduction in the bodys natural Glutathione levels
Rhodes, R
Normally, most laboratory-scale purifications are performed with affinity-tagged proteins