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After each experimental run, CCD images were analyzed frame-by-frame to determine the freezing temperature of each nucleation event, determined by the opacity of the droplet, where liquid droplets were transparent and frozen droplets were opaque

C., Vigo, E

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Brain slice immunofluorescence Mice were anesthetized with ketamine/xylazine 80/5 mg/kg (i.p.) and transcardially perfused with ice-cold PBS followed by 4% PFA in PBS

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