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Proteins were transferred onto nitrocellulose membranes using a wet transfer apparatus (Bio-Rad) at 100 V for 1 h

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We performed this subgroup analysis for two reasons: 1) to eliminate the potentially confounding factor of using other diabetic medications such as metformin or insulin, and 2) we did not have data on the severity of diabetes such as hemoglobin A1c which may be an additional confounding factor