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A Waters Symmetry reverse-phase C18 column (2.1 mm 150 mm, 3.5 m) was used for the LC separation as described ( 8 and PGB 2 ] were monitored

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This mechanism was elucidated by McCoy et al

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When oxygen availability is limited, glucose is the most metabolically efficient fuel, and during myocardial ischemia, the ratio of acetyl-CoA/CoA in mitochondria increases, leading to the activation of pyruvate dehydrogenase (PDH) kinase, which in turn phosphorylates PDH to inhibit its activity