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Purified PCR product was either sent for sequencing at the NAPCore Facility at the Childrens Hospital of Philadelphia (Philadelphia, PA) or cloned using the Strataclone kit (Agilent, Santa Clara, CA), transformed, screened for the inserted DNA and sequenced

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A total of 94 mature oocytes were cultured, and with the addition of 1.0 mM GSH were matured and divided into 48 reaching the cleavage stage (51.65.8)%, 29 developing into morulas (30.93.7)%, and 20 forming blastocysts (21.21.0)%

For an easy-to-measure concentration of 10mg/mL, you would use 10mL of bacteriostatic water

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Ergothionine: It's Chemistry and Biological Significance, in Recent Advances On Molecules Of Chemical And Biological Importance (A Ugc Sponsored National Seminar), Municipal College, Rourkela, February 6, 2018