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After 7 days, cells were washed with PBS, fixed with 4% paraformaldehyde for 15 min, and stained with alcian blue 8GX solution for 30 min at RT to detect intracellular glycosaminoglycan (GAG)

The rats were young, mature, and old

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UV exposure (1520 minutes natural sunlight or 1012 minutes UVA tanning) within 46 hours post-injection significantly enhances melanogenesis versus MT2 alone