glutathione elution buffer recipe Problem with GST-fusion protein elution- Can anyone help? What's the Binding Capacity of – Why using "reduced" glutathione in

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GSH is predominantly in its thiol-reduced form inside the cells, except in the lumen of the endoplasmic reticulum where it exists only in its GSSG form (Figures 2, 4)

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doi: 10.1016/j.psym.2013.11.001