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One hundred microliters of platelet suspensions were kept to analyze platelet activation by flow cytometry

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The fluorescence signal was measured at the end of each extension step

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The filter was washed two times with 100 L of UA, centrifuged at 14,000 g for 15 min, and incubated with 100 L of IAA solution (0.05 M iodoacetamide in UA) for 20 min in the dark