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If a validated research protocol requires frozen solution storage, aliquots should be prepared under controlled conditions so each portion is thawed once rather than repeatedly freezing and thawing the same vial

Sodium: Add one quarter to one half teaspoon of salt to water or food daily, or use electrolyte drinks

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Ghittoni R , Patrussi L , Pirozzi K , et al

1 Morphine was used based on the consideration that it represents the gold standard for potent analgesia