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Detection of ADP from Cp1B assay The reaction mixtures (200 l) in 100 mM Tris-HCl buffer (pH 8.0) contained 0.25 M Cp1B, 10 mM ATP, 12 mM MgCl 2 , 300 M reduced nicotinamide adenine dinucleotide (NADH), 500 M phosphoenolpyruvic acid (PEP), 41 U per ml pyruvate kinase (PK
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