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For 24 hours before and after each session, do not consume coffee or alcohol

i) LASSS-treated HGF, at as low as 1 ng/ml that is below 2.510 ng/ml of the optimal final-concentration range in culture media (supplementary Figure 1a, left light-blue bar ), activates satellite cells to the maximum level presented by the positive control ( black bar PC , 5 ng/ml HGF)

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35, 68 These results show that ERK is one of the important signal cascades mediating cell proliferation of PSCs