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The eureka moment, as Habener puts it, was when they discovered that in the larger precursor protein proglucagon, there was a signal peptide, an intervening peptide, a peptide that was homologous to mammalian glucagon, and then a second GLP-related peptide later revealed to be GLP-1

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Slides were stained with routine hematoxylin and eosin dye using an IHC automated slide stainer (Leica Biosystems)

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