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Cells were treated in triplicate with a serial dilution of IGF-1 LR3 for 4 hours

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Libraries were quantified with NuQuant, quality checked using a TapeStation instrument (Agilent Technologies) and subjected to 52 bp paired-end sequencing on the NovaSeq 6000 system (Illumina)

Mechanistic studies suggest that anabolicandrogenic steroids impair mitochondrial -oxidation and deplete intracellular glutathione stores, resulting in lipid peroxidation and activation of apoptotic signalling pathways

Someone carrying extra weight who still runs half marathons, with normal blood sugar, normal labs, and good energy, has what the commission calls preclinical obesity: a state worth watching, with elevated future risk, but not necessarily one that demands aggressive treatment today