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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 20% methanol in dichloromethane to produce SS(CAM- d 1 5TPP) 2 (104 mg, 55% from SS(CAM-NHS) 2 ) as a beige fluffy/foamy solid

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doi: 10.2337/diacare.27.11.2628 213 DeFronzoRARatnerREHanJKimDDFinemanMSBaronAD