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In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2

TB-500 is a synthetic version of Thymosin Beta-4 and is commonly explored for its potential systemic effects on healing, cellular migration, and overall recovery