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Initial aliquots of ferrostatin-1, deferoxamine, and ML162 were generous gifts of the Dixon Lab (Stanford) and additional quantities were purchased from Cayman Chemical
This study, involving human participants, was conducted in accordance with the ethical standards of the Declaration of Helsinki
USP9X suppresses ferroptosis in diabetic kidney disease by deubiquitinating Nrf2 in vitro
Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
