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The effect of MOTS-c on OPG and RANKL expression was also associated with AMP-activated protein kinase (AMPK) activation, as the inhibition of AMPK phosphorylation downregulated OPG levels and upregulated RANKL levels
Mice were fed either chow (Altromin #1324, energy density 2.85 kcal/g, Brogaarden, Lynge, Denmark) or a high-fat diet (60% kcal fat, #D12492, energy density 5.21 kcal/g, Ssniff, Soest, Germany) for 35 weeks prior to drug treatment
Both medications suppress appetite, cause gastrointestinal side effects, and lead to rapid weight loss, so the mechanisms underlying muscle pain are similar
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The immune infiltration analysis was performed using the Cibersort algorithm to calculate the degree of infiltration of 22 types of immune cells between the healthy group and the influenza group 21
