After that, the protein samples were purified by ODS C18 SPE column (Agilent) and analyzed by LC-MS/MS, equipped with an EASY-nLC 1200 HPLC system and Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific)
ChaC1 and ChaC2 use the -amine of L-glutamyl residues to cleave the amide bond by transamination, releasing it as a cyclic 5-oxo-l-proline and cysteine glycine dipeptide
Each vial contains0.9% benzyl alcohol, a preservative that inhibits bacterial growth, ensuringsafe multi-dose use
FIGURE 5 Limitations A technical limitation of GluCEST, as with many MRI methods, is its molecular specificity
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Injection into the bladder was also categorized during this work