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Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail
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Meal schedule dependent experiment Based on the results, we selected the 1 g/kg dose of Ex which reduced food intake without observable side effects, and went on to investigate the hypothesised meal schedule dependent effects of Ex in all four possible meal schedule conditions (see Fig
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