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Avoid drinking too much alcohol (8-15 drinks per week or 4-5 drinks over 2-3 hours, depending on your size and gender)

qPCR analysis qPCR for selected wheat genes was conducted with the iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) on a BioRad-iCycler detection system as described previously (Liu et al., 2010)

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Detailed guidance on how to calculate peptide dosages provides step-by-step instructions for manual calculation methods

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