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Ensure you store the vial properly between uses and use it within the expiration window specified by your pharmacy (typically 28 to 56 days after first puncture)

Supporting a dominant role for diet composition in establishing the Firmicutes : Bacteroidetes ratio, previous work has shown that mice maintained on a high-fat diet had a similar cecal Firmicutes : Bacteroidetes ratio to those maintained on a high-carbohydrate diet 77

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doi: 10.1093/jn/nxy183

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If the solution remains cloudy or has persistent particulates after ten minutes of gentle mixing, do not use it