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For Nissl staining, the sections were incubated in pre-warmed 0.1% cresyl violet in acetate buffer (pH 3.6), for 15 min before being dehydrated and treated with differential medium (equal parts ethyl alcohol, ether and chloroform)

Conclusion Obesity is a complex and challenging condition, but with the right tools and training, healthcare professionals can make a profound difference in their patients lives