Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)
Anal Chim Acta 794:9098 Guilbault GG (2020) Fluorescence in enzymology
10.1016/0041-008X(90)90319-P Toxicol
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Importantly, results also demonstrated that the enhancement effect of LASSS on the c-met binding-affinity of HGF is consistently maintained, even after the removal of un-reacted free LASSS (and any degradation products of LASSS), by an interaction between HGF and LASSS or potentially by specific chemical modification of HGF at a high molar ratio of 1:8000 to HGF (over 1:4000)