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Amaranthus uses physiological strategies such as reduced transpiration, increased water-use efficiency (WUE), osmotic adjustment via proline and raffinose oligosaccharides, and antioxidant accumulation (Gonzlez-Rodrguez et al., 2019

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Cys 2 ), cystine-free (Cys 2 -free), or cystine-free/GSH-supplemented (750 M) medium for the indicated timepoints, and cell numbers were determined (n = 2 technical replicates representative of one experiment)

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In an assay mixture 100 g protein was incubated in a 1 ml solution containing 5 mM manganese chloride or MnCl 2 , 0.1 mm DTT, 0.1 mM p-coumaric acid, 0.05 mM NADP(H), 30 mM sodium acetate buffer (pH 7.5) and incubated under dark