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9 The resulting oxidized enzyme is fully inactive

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The reduced precursors NRH and NMNH show promise in overcoming this, although preclinical evidence for health benefits is still limited, and whether their efficacy on functional readouts would be superior to the classic counterparts NR and NMN is also unclear

[4] [6] The compounds ability to regenerate other antioxidants, such as vitamin E, and its involvement in iron absorption further illustrate its multifaceted roles in human physiology

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STD-NMR and TR-NOESY For STD-NMR spectra, protein buffer was exchanged against an NMR buffer with 20 mM HEPES pH 7.5, 100 mM KCl, 2 mM EDTA, 1 mM DTT, 66 mM NDSB-195, D 2 0 (10%), 10% glycerol using two cycles of 10-fold dilution, followed by concentration on Amicon Ultra (regenerated cellulose 3kDa, Millipore, Ireland) at 4000 g for 60 min