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Before starting GLP-1 therapy , patients should discuss their complete medical history with their healthcare provider, including the duration and stability of their Hashimoto's disease, current levothyroxine dose, most recent thyroid function tests, and any family history of thyroid cancer or MEN 2 syndrome

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The proteins were purified from cell culture supernatant using a human albumin affinity column, followed by size exclusion chromatography (SEC), which yielded monomeric fractions that migrated on non-reducing sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels according to their molecular weight (MW) (66.5 kDa) (Fig