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Cloning of Arabidopsis thaliana glutathione synthetase (GSH2) by functional complementation of a yeast gsh2 mutant

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The two anti-insulin antibodies were mixed in a 1:2 donor (cryptate)/acceptor (XL665) ratio in the insulin assay detection buffer (Cisbio Bioassays) and 15 L of the insulin antibody mix was added per well

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