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To prove the success of crossings the presence of transgenes ( nahG , SAT , OASTL , GSH1 , and PCS ) originating from different parents was verified in F 1 generation individuals by polymerase chain reaction (PCR) with primers described earlier ( PpnahG , EcSAT , EcOASTL , EcGSH1 and SpPCS , Wawrzyski et al., 2006)

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