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In case of Drosophila , neuronal redox balance was altered by driving CatalaseRNAi or PHGpxRNAi, along with roGFP by elav-Gal4 to deplete the respective antioxidant enzymes and express the redox biosensor

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Then, the medium was changed to fresh DMEM containing DCFH-DA (10 M) and Hoechst 33342 for ROS analysis and nuclei stain

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