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The patient might suffer from a biliary colic may before or after the episode of acute pancreatitis and associated symptoms are agitation, nausea, and vomiting
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After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C
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