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Its a harmless and temporary effect of the methylene blue being processed by your body

The Sec within catalytic motif serves as the active site

doi: 10.1002/fes3.108 9 CastresanaJ

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(This is necessary to bring the Sample Extract and Calibrators to near neutral pH before addition of 4-VP scavenger reagent Add 10 L diluted 4-VP (525 mM), mix

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