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Synthesis of 2 20K PEGylated AD-114 was prepared via a similar method with the following exceptions: AD-114-Im7-FH in PBS (1.48 mg/mL, 8.1 mL) was added to 2 20K PEG-bis-sulfone reagent ( Supplementary S4 ), then, following overnight conjugation, purified over a HiLoad Superdex 200 column (GE Healthcare #171071-01) using PBS

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General GHK-Cu Dosage Guidelines Typical clinical protocols may include: 12 mg daily Duration: 48 weeks Administered through subcutaneous injection The ideal ghk-cu peptide dosage injection plan depends on factors such as: Treatment goals Skin condition Age Recovery needs Combination peptide protocols Medical history Some advanced protocols also discuss ghk-cu peptide injection dosage 100mg vials, although actual daily administration is carefully measured in smaller therapeutic amounts