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Russell-Jones D, Vaag A, Schmitz O, Sethi BK, Lalic N, Antic S, Zdravkovic M, Ravn GM, Sim R

Data-dependent MS2 fragmentation for compound identification and annotation was performed via the AquireX workflow (Thermo Scientific), which comprised three deep scans with MS1 resolution at 60,000 and MS2 resolution at 15,000

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Consistent with the cell cycle and proliferation results, both HNF4-depleted HepG2 cells and SNU449 cells displayed reduced protein synthesis compared to control HepG2 cells, with reduced intensities of puromycin-labeled peptides based on the SUnSET assay 54 (Supplementary Fig