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Named after the famous Hershey Kisses chocolate because it was discovered in Hershey, Pennsylvania, kisspeptin is a neuropeptide naturally produced in the hypothalamus that plays a crucial role in regulating reproduction
On the experiment day, 100 mM IBMX (3-isobutyl-1-methylxanthine) was prepared by dissolving 22.2 mg in 100% DMSO and diluted in DMEM D1145 to a final concentration of 500 M
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General considerations include: Storing bac water in a clean, controlled environment Protecting containers from excessive heat and direct light Maintaining product packaging until use Following all manufacturer storage recommendations Recording storage conditions when required by laboratory protocols The FDA notes that sterile products should be handled using appropriate aseptic procedures and stored according to manufacturer guidance throughout their period of use
P values are two-sided and not adjusted for multiplicity
Practically yes, vials only hold about 3mL safely, and beyond that you risk overflow
