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After centrifugation, liver protein supernatant was incubated with reaction mixture containing 32 mmol/L homoserine, 0.1 mmol/L pyridoxalphosphate, 7.5 mmol/L 2-mercaptoethanol, 7.0 mmol/L EDTA and 0.1 mol/L PBS (pH 7.4)

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463,464 Additionally, decreased thalamocortical functional connectivity observed through resting-state functional MRI correlates with PDPN