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For immunostaining, cells were permeabilized for 1 min using ice-cold PBS supplemented with 0.1% Triton X-100, blocked for 30 min with PBS supplemented with 1% BSA, incubated with the primary antibody in blocking buffer for 2 h at room temperature (RT), washed and subsequently incubated with secondary antibodies and DAPI in blocking buffer for 2 h at RT
PubMed Central , Accessed 23 Apr
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This type of messaging could actually widen audiences to previously untapped segments, he added
Mutation analysis for GNA11 or GNAQ (commonly positive in blue nevi) may also assist in distinguishing BN from other pigmented melanocytic or soft tissue tumors
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