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After addition of 250 l of RPMI-1640 medium, 300 U/ml penicillin, and 175 g/ml streptomycin, parasites were incubated at 37 C in 5% CO 2 atmosphere for 4 d

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Electrolyte balance also mattersadd a pinch of salt to your water or use a sugar-free electrolyte tablet to replenish sodium and potassium lost during appetite suppression and reduced food intake

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