In this study, embryonic stem cells were transfected with a dominant-negative N-cadherin mutant (NCAD delta) under the control of the Villin promoter, which is active primarily in intestinal epithelial cells, and then were introduced into C57BL/6 mouse blastocysts
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PMID 8017933
Longevity and anti-aging protocols For general anti-aging applications, the standard 5 mg daily for 20 days, repeated once or twice annually, represents the most validated approach
Since the tissue retains excess water after staining, dehydrate the sections by immersing them in absolute ethanol for 1 min

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