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The fluorescence intensity of each protein was calculated as the product of the average fluorescence intensity and fluorescence area
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Soluble His-tagged proteins were purified with NiNTA (Qiagen) as previously 56 , and the eluted proteins were dialyzed against buffer H (20 mM TrisHCl, pH 8.0, 100 mM NaCl, 10% glycerol, and 1 mM MgCl 2 )
Esta activacin acelera el proceso de cicatrizacin al favorecer el desplazamiento de los fibroblastos y las clulas endoteliales hacia la zona lesionada
instead, it often enhances their function
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