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Site-directed mutagenesis Point mutations were introduced by PCR with Pfu polymerase (Promega) using the double stop-codon construct of pQE30/ SCGRX7 (ref

The relative usage of these sites is regulated in a cell cycle- and cell type-dependent manner [8, 19, 20]

To provide a more detailed characterization of lymphocytes antibody staining was used to quantify the percentage of T-helper (CD4 + ), cytotoxic T-cells (CD8 + ), memory- (CD45 + ) and regulatory- (CD25 + ) T-cells as well as B-cells (CD21 + ) and monocytes (CD14 + )

Radiation inactivation of microsomal glutathione S-transferase