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The excision of the SAT1 flipper cassette was induced by inoculating 5 mL of YPD broth, containing 2% maltose (without nourseothricin), with the nourseothricin-resistant strains and incubating them in a rotary shaker at 30 C overnight

In SURMOUNT-1, the most common adverse events at the 15 mg dose included nausea (31%), diarrhea (23%), constipation (11%), and vomiting (12%)

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Add 50 L of Colorimetric Solution to each well and mix briefly

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